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After the treatment, the area will feel similar to light sunburn and the pigmented lesions will get darker and sometimes red

The first method involved increasing glutathione levels in SA-deficient NahG and wild type control Xanthi tobaccos by infiltrating intact leaf halves of plants with aqueous solutions of 2 mM reduced glutathione (GSH) or R-2-oxothiazolidine-4-carboxylic acid (OTC) (Sigma-Aldrich, USA) with a hypodermic syringe ( 1 generation individuals for further experiments

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