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Although at least low-level aneuploidy was consistently found in early disease, with recurrent LOH in chromosomes 9 and 10, most sites of metastatic and end-stage disease were affected by aneuploidy due to both WGD and gain and loss of individual chromosomes or chromosome arms

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A critical component was the establishment of an in vitro co-culture system using 12-well plates and 0.4 m pore size transwell inserts

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However, they are not stand-alone solutions