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28.9% women) with type 2 diabetes and atherosclerotic cardiovascular disease and/or chronic kidney disease, of whom 23.1% had a history of HF at baseline (10.3% with HFpEF, 6.1% with HFrEF and 6.7% with unknown subtype)

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Key Factors: Fatty acid side chains (lipophilic modification) Non-natural amino acid substitutions Impact: These modifications: Alter peptide fragmentation behavior Reduce predictability in MS/MS spectra Complicate sequence confirmation and data interpretation 2