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Supplementary material The Supplementary Material for this article can be found online at: Abbreviations AQ amodiaquine AQ-SG 5-glutathion- S -yl-amodiaquine AQ-QI amodiaquine-quinoneimine BSO L -buthionine-sulfoximine CDNB 1-chloro-2, 4-dinitrobenzene CYP cytochromes P450 DEAQ N -desethylamodiaquine DEAQ-SG 5-glutathion- S -yl- N -desethylamodiaquine DEAQ-QI N -desethylamodiaquine-quinoneimine DMEM Dulbeccos modified Eagle medium DMF N,N -dimethylformamide ER endoplasmic reticulum FBS fetal bovine serum GSH glutathione GSSG glutathione disulfide GST glutathione S -transferase H 2 O 2 hydrogen peroxide HClO 4 perchloric acid HRP horseradish peroxidase NQO1 NAD(P)H:quinone oxidoreductase 1 NAPQI N -acetyl- p -benzoquinonimine PBS phosphate-buffered saline QI quinoneimine
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We note that if a suppressed measure is not reported, a MIPS eligible clinician, eligible hospital, and CAH would fail the measure requirement resulting in the MIPS eligible clinician, eligible hospital, and CAH receiving zero points for the suppressed measure or zero points for an objective in which the suppressed measure is one of a cohort of required measures to report due to not meeting the requirement to report the suppressed measure
To further confirm the direct binding of ACE to PCBP1/2, we overexpressed wild-type PCBP1/2, the Cys54 mutant PCBP1, the Cys293 mutant PCBP1, and the Cys54 mutant PCBP2 with a GFP tag and subsequently analyzed direct binding via microtiter thermophoresis (MST)
