After washing with TBST, membranes were incubated for 1 h at room temperature with the secondary antibodies conjugated to horseradish peroxidase (HRP) anti-rabbit IgG (Cell Signaling) or anti-mouse IgG (Cell Signaling)
Cell cycle assay and apoptosis determination Cells were plated at a final concentration of 3 10 5 cells per well in DMEM supplemented with 10 % FBS
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These findings are entirely consistent with data obtained by other investigators in vivo in the mouse and in vitro using isolated mouse hepatocytes (Rashed et al
Similarly, the human papillomavirus (HPV) vaccine can activate the immune system via adjuvants (e.g., aluminum)
