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PMID 16981011

10.1073/pnas.0408624102 Proc

Academic Press, New York, pp 680684 Burton K 1956 A study of the conditions and mechanisms of the diphenylamine reaction for the colorimetric estimation of deoxyribonucleic acid

Alternatively, if there is still quite a bit of your protein in the flow-through then you could use even more resin next time to capture more of your target protein (Figure 5, right)

The thymosin fragment also contains zero cysteine residues , eliminating the possibility of disulfide bond formation, thiol-copper coordination, or copper-catalyzed cysteine oxidation