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The slide images were captured by an inverted microscope and the average optical density (AOD) was measured using Image J 1.53t software

Thymosin beta 4 is found in elevated concentrations in areas where tissue has been damaged

doi: 10.1152/physrev.00003.2008 94 FreskoIHamuryudanVDemirMHizliNSaymanHMelikoluMet al

Health 15 , 100838 (2021)

M., ElMesery M., ElKaref A., Eissa L

These unique biological characteristics not only clearly differentiate ferroptosis from other known cell death forms but also strongly suggest its potential key pathogenic role in various chronic diseases including OA, positioning it as a promising drug intervention target (Dixon et al., 2012