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43 Briefly, double-immunofluorescent staining for GLP-1R and GFAP (staining quiescent PSCs), type I collagen/ -SMA (staining activated PSCs) was performed as previously described

Matched to its certificate of analysis before it s packed

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Pyrosequencing study of fecal microflora of autistic and control children

Product Features Strip plates and additional reagents allow for use in multiple experiments Quantitative protein detection Establishes normal range The best products for confirmation of antibody array data Kit Components Pre-Coated 96-well Strip Microplate Assay Diluent Lyophilized Standard Positive Control Capture Antibody HRP-conjugated secondary antibody Wash Buffer TMB One-Step Substrate Reagent Stop Solution Additional Materials Required Microplate reader capable of measuring absorbance at 450 nm Precision pipettes to deliver 2 L to 1 mL volumes Adjustable 1-25 mL pipettes for reagent preparation 100 mL and 1-liter graduated cylinders Distilled or deionized water Tubes to prepare standard or sample dilutions Orbital shaker Software which can perform four-parameter logistic regression models Assay Procedure Summary Prepare all reagents, samples and standards as instructed