Blocked and permeabilized sections were then incubated overnight at room temperature with rabbit anti-GABA A R-1 (1:500, EMD Millipore, Billerica, MA, catalog# 06868)
For example, take ibuprofen at 8:00 AM, Tylenol at 11:00 AM, and ibuprofen again at 2:00 PM
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Immunoblotting Cells were washed with PBS at the end of treatments if applicable, and then lysed with an appropriate volume of 2X Laemmli sample buffer (Bio-Rad Laboratories, Hercules, CA, USA) with 5% -mercaptoethanol (Bio-Rad Laboratories)