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Participants did not have diabetes
Western blot Cells were lysed in 1 RIPA buffer (Thermo Scientific, 89900) supplemented with cOmplete mini EDTA-free protease inhibitor tablets (Roche, 04693159001) and PhosSTOP phosphatase inhibitor tablets (Roche, 04906837001) and incubated on ice for 10 min
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For tendon, ligament, or nerve targets, it is common to feel little or nothing this early, and that is expected given how slowly those tissues respond
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