We also found that ic ODT8-SST or an sst 2 agonist, unlike selective sst 1 or sst 4 agonists, restored plasma levels of the orexigenic hormone, acyl ghrelin (Hosoda et al., 2002) that were inhibited by abdominal surgery (Stengel et al., 2011b)
After 12 h of transfection, 1 mM sodium butyrate was added, followed by an additional 48 h of incubation
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The samples were evaluated using fluorescence microscopy (Eclipse 80i, Nikon Instruments Europe, Amsterdam, The Netherlands) at 20 magnification
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