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doi:10.3390/ijms19071987 PMC6073405 Wang Y, Wang M, Xiao S, Pan P, Li P, Huo J

Additional authors contributed to specific experiments, data analysis, and manuscript preparation

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Sperm suspensions were then homogenised by careful flicking of the Eppendorf tube, and a sub-sample was diluted 1:4 in milli-Q water (10L of thawed treatment sample in 40L of milli-Q water) to activate sperm by lowering osmolality