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Library Preparation and Sequencing (1) Amplicon Purification PCR products ( 550 bp) were size-verified by 2% agarose gel electrophoresis and purified (TIANgel Midi Purification Kit, Tiangen, DP209, China)

Challenging/Reintroduction: If symptoms improve, foods are slowly reintroduced one at a time over a few days, while carefully monitoring for the return of symptoms

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Owing to their capacity to carry both lipophilic and hydrophilic compounds, eggs are increasingly recognized as effective vehicles for dietary bioactives (5)

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Incorporate practices such as mindfulness, yoga, and deep breathing