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NC9067567) per manufacturers recommendations

doi: 10.1038/451781a

Then, the cells were subjected to immunofluorescence staining for -SMA, desmin, GFAP, and vimentin (Supplementary Table 2) by an overnight incubation with primary antibodies at 4 C, followed by a 1-h incubation with secondary antibody (1:500, Molecular Probes) after a wash step

doi: 10.1097/01.gco.0000193003.58158.4e