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In Pinus spp, enhanced freezing tolerance during cold acclimation was characterized by elevated levels of APX, GR, MDHAR, and DHAR (Tao et al., 1998)

Sections were dewaxed and stained with a rabbit polyclonal anti-FITC (711900, Thermo Fisher Scientific, 1/100) plus secondary Alexa Fluor 488 antibody (Life Technologies, 1/200), and guinea pig anti-human insulin (A0564, Dako, 1/1000) plus secondary Alexa Fluor 546 antibody (Life Technologies, 1/1000) prior to confocal imaging as above

The theoretical extension of this rationale to retatrutide is scientifically plausible but remains exploratory

doi: 10.1016/j.arr.2010.02.005

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