In addition to the tumorigenic effects of succinate accumulation, SDH5 mutation is the key driver supporting the acquisition of epithelialmesenchymal transition (EMT) characteristics
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Sulaiman MA, Al-Farsi YM, Al-Khaduri MM, Saleh J, Waly MI
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Cells were spun down at 3000 g for 5 min, the supernatant was discarded, the pellet was resuspended in 30 mL cold H 2 O and spun down as before described, supernatant was discarded
