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For ghk-cu topical, the first practical step is to separate human evidence from preclinical or anecdotal claims

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2.10 Overexpression and knockdown of RRM2 gene in TPC-1 and IHH4 cells For upregulating RRM2 expression in cells, we obtained a full-length RRM2 sequence fragment using a PCR method and subsequentlycloned it into vector LV18, verified by DNA sequencing and transfected 293T cells (see Supplementary Materials for details)

In: Postharvest Biology and Nanotechnology