For fluorescence staining, we incubated samples for 2 h with Alexa-488 and Alexa-594 conjugated donkey antibodies (1:200) against mouse and rabbit immunoglobulins (Supplementary Figs 6 and 7 are representative images from two sets of immunolabelled cultures of NPCs and neurons)
In the ELIXA study, 6,068 patients with T2D who had an MI or who had been hospitalized for unstable angina within the previous 180 days were randomized to receive lixisenatide or placebo in addition to baseline treatment
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