Inject Water SLOWLY Down the Vial Wall This is the most critical step
PMID: 25990685
At 30 min, 5 h, and 24 h of ligation time, there were marked dilatation and congestion of blood vessels in the portal tracts, central veins, and inusoids, along with the zones of confluent necrosis affecting liver lobuli and the portal tract, which occured at 24 h ligation time
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