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HUBERMAN: Although some people, for whatever reason- will purport that local injections serve recovery of the tissue more readily than systemic injections

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After completion of the isoelectric focusing, the strip was equilibrated in the equilibration solution as described by Barraclough [37] and placed on a polyacrylamide gel for SDS-PAGE with a Protean II unit (Bio-Rad, USA) at a constant voltage (300 V)