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The keloid tissues for organ culture were firstly incubated in a DMEM containing 10% FBS at 37 C for 24 h, and the medium was replaced by the DMEM containing 2% FBS, the final concentration of 25 M FOXO4-DRI was added in the medium immediately
Specifically, EGF has been implicated in stimulating spermatogenesis and reducing germ cell apoptosis [101], while TGF-1 downregulates pro-inflammatory cytokines such as IL-1 [102]