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International Journal of Molecular Sciences, 19 , 954

The peptide is known for its potent ability to stimulate the release of gonadotropin-releasing hormone (GnRH), a crucial factor in the control of the hypothalamic-pituitary-gonadal axis

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3 | Sulfhydryl groups (-SH) measurement Determination -SH wasevaluated according to Ellman'smethod.21 The expe riment is based o n the absorban ce differenc e at 412 nm after photometric reaction between SH and 5,5-dithiobis-(2nitrobenzoic acid)(DTNB).Briefly,colonsupernatantsweremixedwith100Lof SDS(10%)and800Lofphosphatebuffer(10mmol/L,pH8)given the first optical density measurement

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