After washing with TBST, membranes were incubated for 1 h at room temperature with the secondary antibodies conjugated to horseradish peroxidase (HRP) anti-rabbit IgG (Cell Signaling) or anti-mouse IgG (Cell Signaling)
Therefore, melatonin can inhibit the growth of breast cancer cells in the MCF-7 and MDA-MB-231 cell lines by inducing cell death by increasing the expression of the apoptotic markers TUNEL and Bax and the autophagy Beclin-1, LC3, and p62 [37]
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Because of its role as a lipid protector, selenium has been suspected to prevent cardiovascular disease
Control Group ( D ): 125 diabetic patients continued standard anti-diabetic therapy without GSH supplementation
This fact can be used for therapeutic use of this route for substances like glutathione that are poorly absorbed from the gastrointestinal tract