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Brains and serum were collected, and stroke volume and serum IGF-1 levels were quantified (secondary outcomes)

After overnight incubation at 4 C, bound antibodies were visualized using horseradish peroxidase-coupled secondary antibodies and the Clarity Western ECL Substrate (Bio-Rad, 1705061) or Clarity Max ECL western blotting substrate (Bio-Rad, 1705062) for low protein concentrations

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Increased brain lactate during depressive episodes and reversal effects by lithium monotherapy in drug-naive bipolar disorder: a 3-T 1H-MRS study

Clment K, Mosbah H, Poitou C