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A Nrf2 activator, Sulforaphane (SFN), augmented Prdx6, catalase and GST expression in dose-dependent fashion, and halted Nrf2 dysregulation of these antioxidants

The cell debris was removed by centrifugation (45 min, 28,000 g , 4C), and the extracts were stored on ice until use in the enzyme assay and for determination of protein concentration with Bradford Reagent (Sigma-Aldrich, St

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