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The animals were then deeply anesthetized with 3% isoflurane and 50 mg/kg of ketamine followed by a laminectomy at lumbar level L2-L3, corresponding to the sacrocaudal spinal cord

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The formulations were also investigated for their potential for hair growth and hair follicle viability in cases of secondary alopecia

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Post-hoc comparisons were appraised using the conservative Bonferroni/Dunn test