Pickart observed that plasma from older human donors had reduced ability to support cell proliferation in liver cell culture compared to plasma from younger donors, and he set out to identify the factor responsible for the proliferation-supporting activity
Intracellular production of OH from H 2 O 2 is driven by the Fenton reaction, shown in Reaction 3 ( As the most reactive known ROS, OH reacts with nearly all intracellular building blocks and macromolecules (amino acids, nucleic acids, phospholipids, sugars, proteins, RNA, DNA, lipids, polysaccharides)
NF-B activation was measured using QUANTI-Blue solution (Invivogen, rep-qbs)
Actual total body water varies by age, body composition, and health status
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