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Whether to switch to newer generation AEDs needs to be determined on an individual patient basis
Cost and inconsistent insurance coverage also make access difficult
Use tap water or non-sterile liquid to mix
In 96-well round bottom Nunclon Delta plates (Thermo), per well, 90 L of each cell suspension was added to 20 L of antibody solution in PBS and the plates were incubated 6 h at 37 C in a humidified incubator at 5% CO 2
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