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Cells were lysed in 100 L ice cold 8 M Urea/50 mM Tris HCl lysis buffer (Fisher Scientific) with 1:100 of Halt Cocktail Inhibitor, EDTA-free (100X) (Thermo Scientific)

Tirzepatide use in post-bariatric patients requires careful nutritional monitoring, and the B12 in compounded formulations provides valuable but potentially insufficient supplementation

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Complications in Pregnancy Vitamin B12 deficiency contributes to complications in pregnancy