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Then, the cells were washed and suspended in Roswell Park Memorial Institute-1640 medium (RPMI-1640, Sigma-Aldrich) supplemented with 2 g/L sodium bicarbonate, 10% fetal bovine serum (FBS, Sigma-Aldrich), 2% glutamine, and 100 U/mL penicillin-0.1 mg/mL streptomycin (Sigma-Aldrich) and incubated in vitro with RPMI medium (control), tert -Butyl hydroperoxide (tBHP: 200 M) or tBHP plus Ab (22.5 mg/mL) for 30 min at 37C

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Median number of hereditary angioedema attacks was also significantly lower in garadacimab arm versus placebo (0 versus 1.35)

Moreover, full-length BPC157 or single-site alanine-mutated BPC157 was dissolved in HBS-EP Buffer (Cytiva) at concentrations ranging from 1.89 nM to 31.25 nM through serial 2-fold dilutions

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