doi: 10.1530/EC-19-0096
Calidad y confianza en Rankana En Rankana sabemos que buscas lo mejor para tu hogar
Role of the peroxisome proliferator-activated receptor (PPAR) in mediating the effects of brates and fatty acids on gene expression
Apr 2016;87(4):367-72
Oliveira G, Diogo L, Grazina M, Garcia P, Ataide A, Marques C, et al
Briefly, whole Drosophila were fixed in 4% PFA for 12 h at 4 C, then rinsed 3 PBS for 3 h at RT with gentle shaking, and then immersed in FlyClear Solution-1 (8% THEED (2,2,2,2- (Ethylenedinitrilo)-tetraethanol) (Sigma-Aldrich), 5% Triton X-100 (Sigma-Aldrich) and 25% Urea (Fisher Scientific, in PBS) for 14 days at 37 C, as described previously 26