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In summary, clarifying the link between the gut-joint axis and ferroptosis in OA would be of great significance not only for better understanding the pathological processes at multi-levels (local joint tissues, the gut environment, and circulation), but also from a unique angle for guiding novel therapies development in OA Deep investigative approach to understand how major gut bacterial metabolites (tryptophan-derived metabolites, TMAO and so on) modulate ferroptosis in OA is very promising to elucidate the central regulatory mechanism acted by the gut-joint axis on ferroptosis in OA

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Quantitative mass spectrometry-based proteomics: an overview