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Dysbiosis during this transition underlies elevated ketosis risk: the decline of VFA-producing taxaincluding Ruminococcus , Prevotella , Selenomonas , and Succinivibrionaceaeimpairs propionate fermentation pathways and reduces gluconeogenic precursor supply, while the concurrent expansion of opportunistic taxa such as Mycoplasma and Saccharimonas further destabilizes ruminal homeostasis (Table 2), collectively contributing to hypoglycemia, excessive mobilization of body fat, and ketone accumulation, which compromise lactation, reproductive performance, and offspring health 47,55,56,57

Discussion The original method faced several challenges, including the need for strong acids to terminate the enzymatic reaction, which could be hazardous and affect the accuracy of the measurement

& Montavon, M

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Uptake, and subsequent efflux, of l-glutamine is required for the activation of mTOR by essential amino acids, leading to tissue growth ( Adenosine monophosphate-activated protein kinase Further regulation of protein metabolism occurs via adenosine monophosphate-activated protein kinase (AMPK), a sensor of adenosine nucleotides