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Patch-clamp recordings Whole-cell voltage-clamp and cell-attached patch-clamp recordings were conducted at room temperature to measure capsaicin (CAP)- or proton-induced currents in dissociated DRG neurons and hTRPV1-expressing CHO K1 cells, respectively

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To accustom mice to water deprivation schedule, male C57BL/6J lean mice were allowed to access to two water bottles for 2 h (10:0012:00) for 5 days