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If there is presence of enzymatic activity and/or spontaneous oxidation in storage, we would expect to observe lower levels of total GSH and free GSH, and elevated levels of GSSG, in the EDTA-treated sample stored at 4 o C compared to the non-deproteinized EDTA-treated plasma stored at 80 o C

Along with that, ageing would lower the number of nephrons which consequently diminish normal kidney functions by affecting glomerular filtration rate, excess uric acid production, accumulation of creatinine in blood and loss of total kidney function [54,55]

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