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These assays can include determining enzyme kinetics, such as calculating Km and Vmax values, assessing substrate specificity, and evaluating the effects of inhibitors or activators

2023;16:eabp9020

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TGR, human TrxR1 and BmTrxR (all at 4 nM) and PfTrxR (50 nM) were preincubated with the compounds for 15 min

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Material and methodology Plant material and growth conditions for Amaranthus seedlings The seeds of two varieties of Amaranthus tricolor , Pusa Kiran (green) and Pusa Lal Chaulai (red), were acquired from the Division of Vegetable Science, Indian Agricultural Research Institute, New Delhi, India