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tb in individuals with T2DM, we first evaluated the levels of GSH, MDA, and various cytokines in their plasma, RBCs, and PBMCs before and after supplementation with L-GSH or placebo

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Cells and cell debris were removed by differential centrifugation (17,000 g for 10 min followed by 32,000 g for 10 min), and five volumes of chilled Tris buffer were added to dilute the sucrose

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Caspase-3 suppression: The "executioner" enzyme of apoptosis was reduced by 42.7%, directly blocking the cell death cascade