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Once cells were proliferating, they were moved up to higher cell volumes and adapted to DMEM cell culture media with 20% dFBS, 50 U ml 1 penicillin and streptomycin and 1 mM sodium pyruvate for at least 1 week before experimental setup

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However, it is important to consult with a healthcare professional before starting any supplementation regimen

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Seregin IV, Kozhevnikova AD (2023) Phytochelatins: sulfur-containing metal(loid)-chelating ligands in plants