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24 Table 12: Mouse oligonucleotides used for RT-qPCR Oligo forward reverse Actin GCTACAGCTTCACCACCACA AAGGAAGGCTGGAAAAGAGC Cth GCACAAATTGTCCACAAACG CGAAGCCGACTATTGAGGTC Cbs GGCACACTCTCTCACATCCT TAGCAGGTCAATGGCAGTGA Fosl1 TGGAGAAAGGGAGATGCAAG GAGACCGACAAATTGGAGGA Atf4 TCGATGCTCTGTTTCGAATG GGCAACCTGGTCGACTTTTACC Mitf GGAACAGCAACGAGCTAAGG TGATGATCCGATTCACCAGA Tyr ATTGATTTTGCCCATGAAGC AAGGATGCTGGGCTGAGTAA Dct AGCAGACGGAACACTGGACT GCATCTGTGGAAGGGTTGTT Mmp3 GTCTTCCGGTCCTGCTGTGGCTGT CAGGTTCCAGAGAGTTAGACTTGGTG 4.1.11'Cloning'and'sequencing'oligonucleotides' Table 13: Oligonucleotides used for cloning of G6PD and sequencing Construct/Oligo Sequence H-flag_G6PD_Nhe1_5 GCGGCTAGCATGGATTACAAGGATGACGACGATAAGGGCCGGCGGGGCTCAGCC H_G6PD_BstBI_3 GCGTTCGAATCAGAGCTTGTGGGGGTTCA T3 (forward primer) ATTAACCCTCACTAAAGGGA T7 (reverse primer) GCCCTATAGTGAGTCGTATTAC TYMV (forward primer) AGATCGCCTGGAGCAATTCC IRES (reverse primer) CACACCGGCCTTATTCCAAG 4.1.12'Genotyping'oligonucleotides' Table 14: Oligonucleotides for mouse genotyping Oligo forward reverse BRAFV600E TGAGTATTTTTGTGGCAACTGC CTCTGCTGGGAAAGCGGC Pten CAAGCACTCTGCGAACTGAG AAGTTTTTGAAGGCAAGATGC Tyr-CreERT2 GCGGTCTGGCAGTAAAAACTATC GTGAAACAGCATTGCTGTCACTT Cth TGCCGACCAATAAGCAGGGC CCGAAGACTGGCCCGGGAGT CCAGACCGGCAACGAAAATCA ' 4!!!!Material!and!Methods

To compensate, the pancreas increases insulin production, but cannot achieve positive results, and a progressive disorder of lipid and glucose metabolism takes place

\t Delayed Return to Fertility: It may take months for fertility to return after stopping

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