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Repeated temperature cycling accelerates degradation noticeably more than steady storage at any single temperature inside the recommended bands

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Quantitative PCR (qPCR) was performed using PowerTrack SYBR Green Master Mix (Applied Biosystems

However, numerous studies have reported that increased intestinal permeability often precedes the onset of T1D-associated symptoms suggesting that disruption of gut barrier integrity may have an aetiological role in T1D or at least contribute to disease pathogenesis (Meddings et al

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Schwingshackl L, Boeing H, Stelmach-Mardas M, Gottschald M, Dietrich S, Hoffmann G, et al