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In our LC-ICP MS experiments K D values (determined by NTA and His) in 5 mM HEPES, 50 mM NaCl (data not shown) were similar to those in 50 mM HEPES, 50 mM NaCl, which allows to conclude that HEPES at 50 mM level does not compete with HSA, NTA and His for Cu(II) binding or ternary HEPES complexes are formed with both CuHSA and CuNTA

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