Generated ROS during MTX administration provoke DNA damage and induce apoptosis via activating intrinsic apoptotic pathways, as documented in various studies (Koppelmann et al., 2012
32 It was documented that oxidative stress, cholinergic insufficiency, and the accumulation of A and NFTs occurred in the brains of rats following oral administration of aluminium at a dosage of 300 mg/kg body weight
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The ROS produced include the superoxide anion (O2) radicals [22], the hydroxyl (OH) radicals , hydrogen peroxide [70] and the highly cytotoxic peroxynitrite (ONOO) anion [71]
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