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This genetic complexity of human diseases limits the broad application of gene editing therapies, except for well-known diseases caused by mutations of a single gene, like sickle cell anemia 14

Effects of 1 year of exercise training versus combined exercise training and weight loss on body composition, low-grade inflammation and lipids in overweight patients with coronary artery disease: a randomized trial

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The above described process is carried out in a solvent optionally in presence of a coupling agent and/or a base