Recent studies on the cellular effects of melatonin have revealed that melatonin primarily acts on various signal transduction systems, such as inhibition of adenosine phosphorus esters and Ca 2+ mobilization, inhibition of arachidonic acid release, activation of protein kinase C, protein C inhibition of adenylyl cyclase, and opening of potassium channels (Campbell et al
Probing position N108, we found that incorporation of any other amino acid resulted in a substantially reduced formation of cyanidin ( 8 ) suggesting that the hydrogen-bond interaction of the asparagine to the flavonoid substrate is critical for dehydratase activity, while GSH-transferase activity was comparatively less affected for N108 variants comprising an alanine, histidine, tyrosine or tryptophan (Fig
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Endocrinology 156 , 14531463 (2015)
Nagar et al., 2018)
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