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(Updated August 17, 2023)

The dark/light cycle were 7am to 7 pm light and 7 pm to 7am dark, ambient temperature was 22 C + /- 2 C and humidity was 4565%

MCF-7 Cas9-expressing cells were transduced in independent triplicate samples of 1.73 10 8 cells each with the lentiviral whole-genome sgRNA library at a multiplicity of infection of 0.25-0.29, as measured by puromycin resistance, to achieve a representation of 252287 cells per sgRNA

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Before starting glutathione supplements, it is important to talk to your doctor about the potential risks and side effects of taking excessive doses over time

Baylor Univ Med Cent Proc