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[0081] 42.6 g of crude Semaglutide was weighed, dissolved in 1500 mL of a mixed solvent of 25% acetonitrile and 75% water, and purified using Waters 2545 RP-HPLC system with a wavelength of 218 nm, a chromatographic column of 50 x 250 mm reverse phase C18 column, a column temperature of 45C, and a conventional 0.1% TFA/acetonitrile mobile phase

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