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Greater cell cycle inhibition and cytotoxicity induced by 2-deoxy-D-glucose in tumor cells treated under hypoxic vs aerobic conditions

For detection of SNIP1 (Thermo Scientific, PA5-29412, 1:1000) and BUD13 (Bethyl, A303-321A, 1:2000) proteins by western blotting, the SF3B1 pull down protocol was modified in a way that six in-vitro kinase reactions were pooled together for each condition (6 times with and 6x without flag-tagged CDK11 kinase)

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The results showed a significant decrease in the binding of FOXO4 to P53 after FOXO4-DRI treatment (Figure 5A)

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doi: 10.1515/hsz-2016-0336 38 PantopoulosKPorwalSKTartakoffADevireddyL