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Spheroids were treated with RSL3 and Fer1 for 48 h

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In line with the RSL3 data, cholesterol rendered HT1080 cells more resistant to RSL3, cysteine starvation, erastin treatment as well as when GPX4 is deleted by CRISPR mediated knockout, with a comparable potency to Fer-1 (ferrostatin-1) (Fig

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Moreover, depletion of polyamines by SSAT significantly inhibits Wnt/-catenin signaling for cell proliferation, migration, and invasion in HCC and CRC (Wang et al., 2017)