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The correct sequence of the GOI was confirmed by the Sanger sequencing service of Microsynth

The proposed mechanisms for tirzepatide's efficacy include: Augmented insulin secretion : GIP receptor activation may complement GLP-1-mediated insulin release, particularly in the postprandial state Potential effects on insulin sensitivity : Emerging evidence suggests GIP signalling in adipose tissue might influence lipid metabolism Weight reduction : Dual agonism appears to produce pronounced effects on appetite suppression Effects on lipid parameters : Early data suggest GIP receptor activation may influence lipid metabolism alongside GLP-1 effects Both tirzepatide and exendin-4-based agents exhibit glucose-dependent mechanisms , meaning insulin secretion is enhanced only when blood glucose levels are elevated

Enzyme-linked immunosorbent assay (ELISA) For the ligandreceptor affinity ELISA, the human FBXO22 protein served as an antigen to coat the 96-well plate at 4 C for 24 h

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