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The following day, cells were treated with Accutase (Sigma) and resuspended in PBS, washed twice with Ca 2+ -free buffer (NaCl 116 mM, KCl 5.6 mM, MgCl 2 1.2 mM, NaHCO 3 5 mM, NaH 2 PO 4 1 mM, HEPES 20 mM, Glucose 1 g/L)

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